Plasmid_Backbone

Part:BBa_K3308093:Design

Designed by: Jemy Vargehse   Group: iGEM19_Pittsburgh   (2019-10-06)


pKLD66 (pTEV6- maltose binding protein) Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1210
    Illegal XbaI site found at 6570
    Illegal PstI site found at 1222
    Illegal PstI site found at 2547
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 1222
    Illegal PstI site found at 2547
    Illegal NotI site found at 1236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 393
    Illegal BglII site found at 6504
    Illegal XhoI site found at 1143
    Illegal XhoI site found at 1245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1210
    Illegal XbaI site found at 6570
    Illegal PstI site found at 1222
    Illegal PstI site found at 2547
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1210
    Illegal XbaI site found at 6570
    Illegal PstI site found at 1222
    Illegal PstI site found at 2547
    Illegal NgoMIV site found at 1544
    Illegal NgoMIV site found at 4724
    Illegal NgoMIV site found at 4884
    Illegal NgoMIV site found at 6472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 91
    Illegal BsaI site found at 2721
    Illegal SapI.rc site found at 3803


Design Notes

solubility of insert proteins, prevents formation of inclusion bodies.


Source

the sequence was found of Uniport

References